Multi-epitope vaccine against cystic echinococcosis using immunodominant epitopes from EgA31 and EgG1Y162 antigens

Multi-epitope vaccine against cystic echinococcosis using immunodominant epitopes from EgA31 and EgG1Y162 antigens

Blog Article

Cystic Decorative Jars echinococcosis (CE) is a near-cosmopolitan public health concern, especially in developing countries.Parasite ova shed via definitive host feces are responsible for livestock and occasionally human infection, leading to hydatid cysts.Immunization using proper antigens is a good immunoprophylactic strategy.This study was aimed to design a multi-epitope vaccine using EgA31 and EgG1Y162 antigens.Top high-ranked B-cell epitopes and major histocompatibility complex (MHC)-binding epitopes were predicted and selected to construct the vaccine model.

Then, physico-chemical features, secondary and tertiary structures, refinement and validations were all evaluated using web servers for the multi-epitope vaccine construct.Finally, non-linear B-cell epitopes were determined for vaccine-antibody Voltage Detectors interactions.Moreover, the vaccine construct was subjected to disulfide engineering, molecular docking with human MHC-I and MHC-II molecules as well as codon adaptation and in silico cloning processes.In conclusion, this multimeric CE vaccine needs experimental and clinical confirmations to be considered as an actually immunogenic vaccine model.